Development of antagonistic yeasts for controlling black mold disease of onion

Abstract Background The present study aimed to examine the biocontrol efficacy of 28 yeast isolates against Aspergillus niger , common pathogen onion black mold disease. antagonistic potential A. growth was investigated using a dual culture technique. Results Five isolates, including 8 and 11, showed highest inhibition effect on mycelial . Molecular analysis 16S RNA identified strains (AUN-AH14) 11 (AUN-AH23) as Galactomyces geotrichum (JQ713185.1) G. (DQ849321.1), respectively. filtrates AUN-AH14 AUN-AH23 at percentage concentrations 20, 40, 60, 80 were tested As result, lowest dry weight obtained with two 60 80%.. Treatment bulbs formulation their corresponding cultural 80% concentration significantly reduced severity disease relative controls. Conclusions most significant reduction in proved formulations AUN-AH23, followed by (CF-AUN-AH14 CF-AUN-AH23). shelf life formulated began gradually deteriorate after 5 months storage 4 °C.